3D cell assembly via anode electrode manipulation

Yajing Shen; Chin To Wong; Wenfeng Wan; Masahiro Nakajima; Toshio Fukuda

doi:10.1109/CYBER.2015.7288104

3D cell assembly via anode electrode manipulation

Yajing Shen^*, Chin To Wong, Wenfeng Wan, Masahiro Nakajima, Toshio Fukuda

^*Corresponding author for this work

Research output: Chapter in Book/Conference Proceeding/Report › Conference Paper published in a book › peer-review

Abstract

Cell culture is a basic and significant task in biological engineering. Many cell culture works have been studied up to now. However, the cell is always cultured in a 2D plate in current techniques. Thus, the cells can only growth on a flat plate, which condition is far from that in vivo. Hydrogel is a kind of biocompatible material, inside with the cell can spread and growth. It provides us a method to study the cell's property in real 3D environment. Thus, how to control the shape of the gel is an important topic. In this paper, we proposed a method to control the gel's shape and position based on the manipulation of the anode electrode. The gel generation is based on the electrodeposition of calcium alginate. The reaction speed is controlled by the applied current. Three examples were demonstrated to show how our system is working. The result shows that straight gel line, arbitrary gel shape on substrate and multi-layer gel structure can be obtained by this proposed method.

Original language	English
Title of host publication	2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1138-1142
Number of pages	5
ISBN (Electronic)	9781479987290
DOIs	https://doi.org/10.1109/CYBER.2015.7288104
Publication status	Published - 2 Oct 2015
Externally published	Yes
Event	5th Annual IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015 - Shenyang, China Duration: 9 Jun 2015 → 12 Jun 2015

Publication series

Name	2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015

Conference

Conference	5th Annual IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015
Country/Territory	China
City	Shenyang
Period	9/06/15 → 12/06/15

Bibliographical note

Publisher Copyright:
© 2015 IEEE.

Access to Document

10.1109/CYBER.2015.7288104

Cite this

Shen, Y., Wong, C. T., Wan, W., Nakajima, M., & Fukuda, T. (2015). 3D cell assembly via anode electrode manipulation. In 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015 (pp. 1138-1142). Article 7288104 (2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/CYBER.2015.7288104

Shen, Yajing ; Wong, Chin To ; Wan, Wenfeng et al. / 3D cell assembly via anode electrode manipulation. 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1138-1142 (2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015).

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title = "3D cell assembly via anode electrode manipulation",

abstract = "Cell culture is a basic and significant task in biological engineering. Many cell culture works have been studied up to now. However, the cell is always cultured in a 2D plate in current techniques. Thus, the cells can only growth on a flat plate, which condition is far from that in vivo. Hydrogel is a kind of biocompatible material, inside with the cell can spread and growth. It provides us a method to study the cell's property in real 3D environment. Thus, how to control the shape of the gel is an important topic. In this paper, we proposed a method to control the gel's shape and position based on the manipulation of the anode electrode. The gel generation is based on the electrodeposition of calcium alginate. The reaction speed is controlled by the applied current. Three examples were demonstrated to show how our system is working. The result shows that straight gel line, arbitrary gel shape on substrate and multi-layer gel structure can be obtained by this proposed method.",

author = "Yajing Shen and Wong, \{Chin To\} and Wenfeng Wan and Masahiro Nakajima and Toshio Fukuda",

note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 5th Annual IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015 ; Conference date: 09-06-2015 Through 12-06-2015",

year = "2015",

month = oct,

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doi = "10.1109/CYBER.2015.7288104",

language = "English",

series = "2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015",

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Shen, Y, Wong, CT, Wan, W, Nakajima, M & Fukuda, T 2015, 3D cell assembly via anode electrode manipulation. in 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015., 7288104, 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1138-1142, 5th Annual IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015, Shenyang, China, 9/06/15. https://doi.org/10.1109/CYBER.2015.7288104

3D cell assembly via anode electrode manipulation. / Shen, Yajing; Wong, Chin To; Wan, Wenfeng et al.
2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1138-1142 7288104 (2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015).

Research output: Chapter in Book/Conference Proceeding/Report › Conference Paper published in a book › peer-review

TY - GEN

T1 - 3D cell assembly via anode electrode manipulation

AU - Shen, Yajing

AU - Wong, Chin To

AU - Wan, Wenfeng

AU - Nakajima, Masahiro

AU - Fukuda, Toshio

PY - 2015/10/2

Y1 - 2015/10/2

N2 - Cell culture is a basic and significant task in biological engineering. Many cell culture works have been studied up to now. However, the cell is always cultured in a 2D plate in current techniques. Thus, the cells can only growth on a flat plate, which condition is far from that in vivo. Hydrogel is a kind of biocompatible material, inside with the cell can spread and growth. It provides us a method to study the cell's property in real 3D environment. Thus, how to control the shape of the gel is an important topic. In this paper, we proposed a method to control the gel's shape and position based on the manipulation of the anode electrode. The gel generation is based on the electrodeposition of calcium alginate. The reaction speed is controlled by the applied current. Three examples were demonstrated to show how our system is working. The result shows that straight gel line, arbitrary gel shape on substrate and multi-layer gel structure can be obtained by this proposed method.

AB - Cell culture is a basic and significant task in biological engineering. Many cell culture works have been studied up to now. However, the cell is always cultured in a 2D plate in current techniques. Thus, the cells can only growth on a flat plate, which condition is far from that in vivo. Hydrogel is a kind of biocompatible material, inside with the cell can spread and growth. It provides us a method to study the cell's property in real 3D environment. Thus, how to control the shape of the gel is an important topic. In this paper, we proposed a method to control the gel's shape and position based on the manipulation of the anode electrode. The gel generation is based on the electrodeposition of calcium alginate. The reaction speed is controlled by the applied current. Three examples were demonstrated to show how our system is working. The result shows that straight gel line, arbitrary gel shape on substrate and multi-layer gel structure can be obtained by this proposed method.

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U2 - 10.1109/CYBER.2015.7288104

DO - 10.1109/CYBER.2015.7288104

M3 - Conference Paper published in a book

T3 - 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015

SP - 1138

EP - 1142

BT - 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 5th Annual IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015

Y2 - 9 June 2015 through 12 June 2015

ER -

Shen Y, Wong CT, Wan W, Nakajima M, Fukuda T. 3D cell assembly via anode electrode manipulation. In 2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1138-1142. 7288104. (2015 IEEE International Conference on Cyber Technology in Automation, Control and Intelligent Systems, IEEE-CYBER 2015). doi: 10.1109/CYBER.2015.7288104

3D cell assembly via anode electrode manipulation

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