A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells

Xinbo Huang; Jieyi Wang; Xia Wu; Bin Jiang; Menghui Chen; Han Chen; Yan Mao; Cheng zhou; Bo Yu

doi:10.1002/ctd2.125

A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells

Xinbo Huang, Jieyi Wang, Xia Wu, Bin Jiang, Menghui Chen, Han Chen, Yan Mao, Cheng zhou, Bo Yu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

1 Citation (Scopus)

Abstract

Optogenetic systems and tetracycline-induced expression systems have been used in biological research. In gene circuits, light and tetracycline are often used to control the activation or inhibition of gene expression. In our study, we used a blue light induction system and tetracycline induction system to construct an “OR” logic gate, which can specifically induce the expression of p53 protein or E-cadherin in the case of blue light or tetracycline, thus inhibiting the growth of cutaneous squamous cell carcinoma cells. Cutaneous squamous cell carcinoma is a kind of skin surface tumour. The activation of two tumour suppressor genes has a synergistic inhibitory effect on cutaneous squamous cell carcinoma, and blue light and tetracycline also provide a flexible means for gene regulation.

Original language	English
Article number	e125
Journal	Clinical and Translational Discovery
Volume	2
Issue number	4
DOIs	https://doi.org/10.1002/ctd2.125
Publication status	Published - Dec 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 The Authors. Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.

Keywords

CRISPR
cutaneous squamous cell carcinoma
light induction system
logic gate
tetracycline induction system

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/ctd2.125

Cite this

Huang, X., Wang, J., Wu, X., Jiang, B., Chen, M., Chen, H., Mao, Y., zhou, C., & Yu, B. (2022). A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells. Clinical and Translational Discovery, 2(4), Article e125. https://doi.org/10.1002/ctd2.125

@article{838fcfdc0f714d0694c5306e809a4a3f,

title = "A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells",

abstract = "Optogenetic systems and tetracycline-induced expression systems have been used in biological research. In gene circuits, light and tetracycline are often used to control the activation or inhibition of gene expression. In our study, we used a blue light induction system and tetracycline induction system to construct an “OR” logic gate, which can specifically induce the expression of p53 protein or E-cadherin in the case of blue light or tetracycline, thus inhibiting the growth of cutaneous squamous cell carcinoma cells. Cutaneous squamous cell carcinoma is a kind of skin surface tumour. The activation of two tumour suppressor genes has a synergistic inhibitory effect on cutaneous squamous cell carcinoma, and blue light and tetracycline also provide a flexible means for gene regulation.",

keywords = "CRISPR, cutaneous squamous cell carcinoma, light induction system, logic gate, tetracycline induction system",

author = "Xinbo Huang and Jieyi Wang and Xia Wu and Bin Jiang and Menghui Chen and Han Chen and Yan Mao and Cheng zhou and Bo Yu",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Clinical and Translational Discovery published by John Wiley \& Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.",

year = "2022",

month = dec,

doi = "10.1002/ctd2.125",

language = "English",

volume = "2",

journal = "Clinical and Translational Discovery",

issn = "2768-0622",

publisher = "John Wiley and Sons Inc",

number = "4",

}

Huang, X, Wang, J, Wu, X, Jiang, B, Chen, M, Chen, H, Mao, Y, zhou, C & Yu, B 2022, 'A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells', Clinical and Translational Discovery, vol. 2, no. 4, e125. https://doi.org/10.1002/ctd2.125

A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells. / Huang, Xinbo; Wang, Jieyi; Wu, Xia et al.
In: Clinical and Translational Discovery, Vol. 2, No. 4, e125, 12.2022.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells

AU - Huang, Xinbo

AU - Wang, Jieyi

AU - Wu, Xia

AU - Jiang, Bin

AU - Chen, Menghui

AU - Chen, Han

AU - Mao, Yan

AU - zhou, Cheng

AU - Yu, Bo

PY - 2022/12

Y1 - 2022/12

N2 - Optogenetic systems and tetracycline-induced expression systems have been used in biological research. In gene circuits, light and tetracycline are often used to control the activation or inhibition of gene expression. In our study, we used a blue light induction system and tetracycline induction system to construct an “OR” logic gate, which can specifically induce the expression of p53 protein or E-cadherin in the case of blue light or tetracycline, thus inhibiting the growth of cutaneous squamous cell carcinoma cells. Cutaneous squamous cell carcinoma is a kind of skin surface tumour. The activation of two tumour suppressor genes has a synergistic inhibitory effect on cutaneous squamous cell carcinoma, and blue light and tetracycline also provide a flexible means for gene regulation.

AB - Optogenetic systems and tetracycline-induced expression systems have been used in biological research. In gene circuits, light and tetracycline are often used to control the activation or inhibition of gene expression. In our study, we used a blue light induction system and tetracycline induction system to construct an “OR” logic gate, which can specifically induce the expression of p53 protein or E-cadherin in the case of blue light or tetracycline, thus inhibiting the growth of cutaneous squamous cell carcinoma cells. Cutaneous squamous cell carcinoma is a kind of skin surface tumour. The activation of two tumour suppressor genes has a synergistic inhibitory effect on cutaneous squamous cell carcinoma, and blue light and tetracycline also provide a flexible means for gene regulation.

KW - CRISPR

KW - cutaneous squamous cell carcinoma

KW - light induction system

KW - logic gate

KW - tetracycline induction system

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001224383500023

UR - https://openalex.org/W4302292962

U2 - 10.1002/ctd2.125

DO - 10.1002/ctd2.125

M3 - Journal Article

SN - 2768-0622

VL - 2

JO - Clinical and Translational Discovery

JF - Clinical and Translational Discovery

IS - 4

M1 - e125

ER -

A logic “OR” gate composed of a blue light-induced CRISPR/dCas9 system and a Tet-on system activates cancer suppressor genes to inhibit the growth of cutaneous squamous cell carcinoma cells

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this