A plant homologue of 36 kDa subunit of replication factor C: Molecular cloning and characterization

Tomoyuki Furukawa; Seisuke Kimura; Toyotaka Ishibashi; Junji Hashimoto; Kengo Sakaguchi

doi:10.1016/S0168-9452(01)00384-3

A plant homologue of 36 kDa subunit of replication factor C: Molecular cloning and characterization

Tomoyuki Furukawa, Seisuke Kimura, Toyotaka Ishibashi, Junji Hashimoto, Kengo Sakaguchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

5 Citations (Scopus)

Abstract

We have isolated a 1.47 kb cDNA from rice (Oryza sativa) encoding plant homologue of a small subunit of Replication Factor C (RFC), and designated OsRFC3. The open reading frame of OsRFC3 encoded a predicted product of 367 amino acid residues with a molecular mass of 40.3 kDa. Genomic analysis indicated that the subunit existed as a single copy per genome. The transcript was highly expressed in root tips that contain root apical meristem, and the young and mature leaves. The mRNA level of OsRFC3 was significantly reduced when the cell proliferation was temporarily halted by removal of sucrose from the growth medium. When the growth-halted cells began to re-grow following addition of sucrose to the medium, OsRFC3 was again expressed at high level. The roles of OsRFC3 in plant DNA replication is discussed.

Original language	English
Pages (from-to)	99-106
Number of pages	8
Journal	Plant Science
Volume	161
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(01)00384-3
Publication status	Published - 2001
Externally published	Yes

Keywords

DNA replication and repair
Oryza sativa L. cv. Nipponbare
OsRFC3
Replication factor C

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10.1016/S0168-9452(01)00384-3

Cite this

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title = "A plant homologue of 36 kDa subunit of replication factor C: Molecular cloning and characterization",

abstract = "We have isolated a 1.47 kb cDNA from rice (Oryza sativa) encoding plant homologue of a small subunit of Replication Factor C (RFC), and designated OsRFC3. The open reading frame of OsRFC3 encoded a predicted product of 367 amino acid residues with a molecular mass of 40.3 kDa. Genomic analysis indicated that the subunit existed as a single copy per genome. The transcript was highly expressed in root tips that contain root apical meristem, and the young and mature leaves. The mRNA level of OsRFC3 was significantly reduced when the cell proliferation was temporarily halted by removal of sucrose from the growth medium. When the growth-halted cells began to re-grow following addition of sucrose to the medium, OsRFC3 was again expressed at high level. The roles of OsRFC3 in plant DNA replication is discussed.",

keywords = "DNA replication and repair, Oryza sativa L. cv. Nipponbare, OsRFC3, Replication factor C",

author = "Tomoyuki Furukawa and Seisuke Kimura and Toyotaka Ishibashi and Junji Hashimoto and Kengo Sakaguchi",

year = "2001",

doi = "10.1016/S0168-9452(01)00384-3",

language = "English",

volume = "161",

pages = "99--106",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - A plant homologue of 36 kDa subunit of replication factor C

T2 - Molecular cloning and characterization

AU - Furukawa, Tomoyuki

AU - Kimura, Seisuke

AU - Ishibashi, Toyotaka

AU - Hashimoto, Junji

AU - Sakaguchi, Kengo

PY - 2001

Y1 - 2001

N2 - We have isolated a 1.47 kb cDNA from rice (Oryza sativa) encoding plant homologue of a small subunit of Replication Factor C (RFC), and designated OsRFC3. The open reading frame of OsRFC3 encoded a predicted product of 367 amino acid residues with a molecular mass of 40.3 kDa. Genomic analysis indicated that the subunit existed as a single copy per genome. The transcript was highly expressed in root tips that contain root apical meristem, and the young and mature leaves. The mRNA level of OsRFC3 was significantly reduced when the cell proliferation was temporarily halted by removal of sucrose from the growth medium. When the growth-halted cells began to re-grow following addition of sucrose to the medium, OsRFC3 was again expressed at high level. The roles of OsRFC3 in plant DNA replication is discussed.

AB - We have isolated a 1.47 kb cDNA from rice (Oryza sativa) encoding plant homologue of a small subunit of Replication Factor C (RFC), and designated OsRFC3. The open reading frame of OsRFC3 encoded a predicted product of 367 amino acid residues with a molecular mass of 40.3 kDa. Genomic analysis indicated that the subunit existed as a single copy per genome. The transcript was highly expressed in root tips that contain root apical meristem, and the young and mature leaves. The mRNA level of OsRFC3 was significantly reduced when the cell proliferation was temporarily halted by removal of sucrose from the growth medium. When the growth-halted cells began to re-grow following addition of sucrose to the medium, OsRFC3 was again expressed at high level. The roles of OsRFC3 in plant DNA replication is discussed.

KW - DNA replication and repair

KW - Oryza sativa L. cv. Nipponbare

KW - OsRFC3

KW - Replication factor C

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000169298300011

UR - https://openalex.org/W2024955593

UR - https://www.scopus.com/pages/publications/0034972709

U2 - 10.1016/S0168-9452(01)00384-3

DO - 10.1016/S0168-9452(01)00384-3

M3 - Journal Article

SN - 0168-9452

VL - 161

SP - 99

EP - 106

JO - Plant Science

JF - Plant Science

IS - 1

ER -

A plant homologue of 36 kDa subunit of replication factor C: Molecular cloning and characterization

Abstract

Keywords

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