Amperometric immunosensor for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) in water

Marianne Wilmer*, Dieter Trau, Reinhard Renneberg, Friedrich Spener

*Corresponding author for this work

Research output: Contribution to journalJournal Articlepeer-review

38 Citations (Scopus)

Abstract

An amperometric immunosensor for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in water has been developed using sequential injection analysis techniques. The system is based on a rapid competitive enzyme immunoassay employing an alkaline phosphatase-labeled monoclonal antibody directed against the herbicide and an immunoreactor with 2,4-D immobilized via bovine serum albumin either to Eupergit in a column or directly to the surface of a glass capillary. The detection limit of the immunosensor at 0.1 μg 2,4-D/1 without enrichment of the analyte makes automatic measurements of 2,4-D in drinking and ground water feasible.

Original languageEnglish
Pages (from-to)515-525
Number of pages11
JournalAnalytical Letters
Volume30
Issue number3
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • 2,4-D
  • Amperometric
  • Drinking water
  • Immunosensor
  • Monoclonal antibodies
  • Phenoxycarbonic acids
  • Sequential injection analysis

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