Abstract
An amperometric immunosensor for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in water has been developed using sequential injection analysis techniques. The system is based on a rapid competitive enzyme immunoassay employing an alkaline phosphatase-labeled monoclonal antibody directed against the herbicide and an immunoreactor with 2,4-D immobilized via bovine serum albumin either to Eupergit in a column or directly to the surface of a glass capillary. The detection limit of the immunosensor at 0.1 μg 2,4-D/1 without enrichment of the analyte makes automatic measurements of 2,4-D in drinking and ground water feasible.
| Original language | English |
|---|---|
| Pages (from-to) | 515-525 |
| Number of pages | 11 |
| Journal | Analytical Letters |
| Volume | 30 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1997 |
| Externally published | Yes |
Keywords
- 2,4-D
- Amperometric
- Drinking water
- Immunosensor
- Monoclonal antibodies
- Phenoxycarbonic acids
- Sequential injection analysis