An oxygen-rich enzyme matrix for biosensor construction based on clark oxygen electrode

Y. Cui*, J. P. Barford, R. Renneberg

*Corresponding author for this work

Research output: Chapter in Book/Conference Proceeding/ReportConference Paper published in a bookpeer-review

Abstract

We report a novel strategy to develop an oxygen-rich enzyme matrix biosensor based on the Clark oxygen electrode. For biosensors based on oxygen detection, the oxygen concentration in the enzyme matrix will significantly affect the biosensor performance. We demonstrate that, by using catalase in the enzyme matrix and hydrogen peroxide in the buffer solution, the concentration of oxygen in the enzyme matrix will be increased significantly. This is due to the release of oxygen from hydrogen peroxide through the enzymatic reaction by catalase. Consequently, the linear detection range will be broadened and the present oxygen-dependence will be alleviated significantly. copyright The Electrochemical Society.

Original languageEnglish
Title of host publicationSensors, Actuators, and Microsystems
PublisherElectrochemical Society Inc.
Pages37-44
Number of pages8
Edition21
ISBN (Electronic)9781566775007
DOIs
Publication statusPublished - 2006
EventSensors, Actuators, and Microsystems - 208th Electrochemical Society Meeting - Los Angeles, CA, United States
Duration: 16 Oct 200521 Oct 2005

Publication series

NameECS Transactions
Number21
Volume1
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

ConferenceSensors, Actuators, and Microsystems - 208th Electrochemical Society Meeting
Country/TerritoryUnited States
CityLos Angeles, CA
Period16/10/0521/10/05

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