TY - JOUR
T1 - Determination of underivatized amino acids by high-performance liquid chromatography and electrochemical detection at an amino acid oxidase immobilized CuPtCI6 modified electrode
AU - Pei, Jianhong
AU - Li, Xiao Yuan
PY - 2000
Y1 - 2000
N2 - An amperometric sensor for amino acids based on the immobilization of amino acid oxidase on the surface of a CuPtCl6/GC is described. The amperometric current is due to the oxidation of H2O2 liberated during the enzyme reaction on the surface of the enzyme electrode. The electrode response characteristics as well as kinetic parameters have been evaluated. The enzyme electrode was characterized as an electrochemical biosensor, which was used as detector in high performance liquid chromatography (HPLC) for the determination of a mixture of amino acids with satisfactory results.
AB - An amperometric sensor for amino acids based on the immobilization of amino acid oxidase on the surface of a CuPtCl6/GC is described. The amperometric current is due to the oxidation of H2O2 liberated during the enzyme reaction on the surface of the enzyme electrode. The electrode response characteristics as well as kinetic parameters have been evaluated. The enzyme electrode was characterized as an electrochemical biosensor, which was used as detector in high performance liquid chromatography (HPLC) for the determination of a mixture of amino acids with satisfactory results.
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000088944900007
UR - https://openalex.org/W2007781878
UR - https://www.scopus.com/pages/publications/0034252876
U2 - 10.1007/s002160000447
DO - 10.1007/s002160000447
M3 - Journal Article
SN - 0937-0633
VL - 367
SP - 707
EP - 713
JO - Fresenius' Journal of Analytical Chemistry
JF - Fresenius' Journal of Analytical Chemistry
IS - 8
ER -