TY - GEN
T1 - Dielectrophoretic (DEP) separation of live/dead cells on a glass slide functionalized with interdigitated 3D silicon ring microelectrodes
AU - Xing, Xiaoxing
AU - Yobas, Levent
PY - 2014
Y1 - 2014
N2 - This paper describes a microfluidic device which offers a dielectrophoretic solution to cell sorting via interdigitated 3D silicon (Si) electrodes on a transparent glass substrate. The Si electrodes with a self-aligned array of lateral rings were fabricated through a single mask process on an anodically bonded Si-glass wafer. The device has been characterized for separation of live/dead human colorectal carcinoma cells at select flow rates and voltages. The live cell capture and dead cell removal efficiencies both reached 90% at an AC activation of 5Vp 450kHz applied against a flow rate up to 0.25ml/hr. The device disclosed here, with successful demonstration of continuous live/dead cell separation, offers great potential as a bioparticle sorting front-end module for a lab-on-a chip system.
AB - This paper describes a microfluidic device which offers a dielectrophoretic solution to cell sorting via interdigitated 3D silicon (Si) electrodes on a transparent glass substrate. The Si electrodes with a self-aligned array of lateral rings were fabricated through a single mask process on an anodically bonded Si-glass wafer. The device has been characterized for separation of live/dead human colorectal carcinoma cells at select flow rates and voltages. The live cell capture and dead cell removal efficiencies both reached 90% at an AC activation of 5Vp 450kHz applied against a flow rate up to 0.25ml/hr. The device disclosed here, with successful demonstration of continuous live/dead cell separation, offers great potential as a bioparticle sorting front-end module for a lab-on-a chip system.
UR - https://openalex.org/W2035369123
UR - https://www.scopus.com/pages/publications/84898978730
U2 - 10.1109/MEMSYS.2014.6765800
DO - 10.1109/MEMSYS.2014.6765800
M3 - Conference Paper published in a book
SN - 9781479935086
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 951
EP - 954
BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Y2 - 26 January 2014 through 30 January 2014
ER -
