DNA kinetics in microfabricated devices

Yick Chuen Chan; Rosie Ming Sum Ma; Maria Carles; Nikolaus J. Sucher; Man Wong; Yitshak Zohar

DNA kinetics in microfabricated devices

Yick Chuen Chan, Rosie Ming Sum Ma, Maria Carles, Nikolaus J. Sucher, Man Wong, Yitshak Zohar^*

^*Corresponding author for this work

Department of Electronic and Computer Engineering

Research output: Contribution to conference › Conference Paper › peer-review

2 Citations (Scopus)

Abstract

The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.

Original language	English
Pages	60-63
Number of pages	4
Publication status	Published - 2002
Event	15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002 - Las Vegas, NV, United States Duration: 20 Jan 2002 → 24 Jan 2002

Conference

Conference	15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002
Country/Territory	United States
City	Las Vegas, NV
Period	20/01/02 → 24/01/02

Cite this

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title = "DNA kinetics in microfabricated devices",

abstract = "The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.",

author = "Chan, \{Yick Chuen\} and Ma, \{Rosie Ming Sum\} and Maria Carles and Sucher, \{Nikolaus J.\} and Man Wong and Yitshak Zohar",

year = "2002",

language = "English",

pages = "60--63",

note = "15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002 ; Conference date: 20-01-2002 Through 24-01-2002",

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TY - CONF

T1 - DNA kinetics in microfabricated devices

AU - Chan, Yick Chuen

AU - Ma, Rosie Ming Sum

AU - Carles, Maria

AU - Sucher, Nikolaus J.

AU - Wong, Man

AU - Zohar, Yitshak

PY - 2002

Y1 - 2002

N2 - The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.

AB - The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.

UR - https://www.scopus.com/pages/publications/0036198280

M3 - Conference Paper

AN - SCOPUS:0036198280

SP - 60

EP - 63

T2 - 15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002

Y2 - 20 January 2002 through 24 January 2002

ER -

DNA kinetics in microfabricated devices

Abstract

Conference

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