Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

De Cai; Terence T.W. Wong; Liren Zhu; Junhui Shi; Sung Liang Chen; Lihong V. Wang

doi:10.1364/OL.43.004875

Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

De Cai, Terence T.W. Wong, Liren Zhu, Junhui Shi, Sung Liang Chen, Lihong V. Wang^*

^*Corresponding author for this work

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Journal Article › peer-review

25 Citations (Scopus)

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

Original language	English
Pages (from-to)	4875-4878
Number of pages	4
Journal	Optics Letters
Volume	43
Issue number	20
DOIs	https://doi.org/10.1364/OL.43.004875
Publication status	Published - 15 Oct 2018

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© 2018 Optical Society of America.

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abstract = "Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.",

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T1 - Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

AU - Cai, De

AU - Wong, Terence T.W.

AU - Zhu, Liren

AU - Shi, Junhui

AU - Chen, Sung Liang

AU - Wang, Lihong V.

PY - 2018/10/15

Y1 - 2018/10/15

N2 - Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

AB - Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.

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DO - 10.1364/OL.43.004875

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VL - 43

SP - 4875

EP - 4878

JO - Optics Letters

JF - Optics Letters

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Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

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