Effect of heparin on phosphorylation site specificity of neuronal Cdc2- like kinase

Zhong Qi; Xiaodong Zhu; Michel Goedert; Donald J. Fujita; Jerry H. Wang

doi:10.1016/S0014-5793(98)00096-9

Effect of heparin on phosphorylation site specificity of neuronal Cdc2- like kinase

Zhong Qi, Xiaodong Zhu, Michel Goedert, Donald J. Fujita, Jerry H. Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

15 Citations (Scopus)

Abstract

Neuronal Cdc2-like kinase (Nclk) can be stimulated by heparin in a substrate-dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9-18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.

Original language	English
Pages (from-to)	227-230
Number of pages	4
Journal	FEBS Letters
Volume	423
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(98)00096-9
Publication status	Published - 20 Feb 1998

Keywords

Alzheimer's disease
Heparin
Neuronal Cdc2-like kinase
Tau

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10.1016/S0014-5793(98)00096-9

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title = "Effect of heparin on phosphorylation site specificity of neuronal Cdc2- like kinase",

abstract = "Neuronal Cdc2-like kinase (Nclk) can be stimulated by heparin in a substrate-dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9-18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.",

keywords = "Alzheimer's disease, Heparin, Neuronal Cdc2-like kinase, Tau",

author = "Zhong Qi and Xiaodong Zhu and Michel Goedert and Fujita, \{Donald J.\} and Wang, \{Jerry H.\}",

year = "1998",

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doi = "10.1016/S0014-5793(98)00096-9",

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T1 - Effect of heparin on phosphorylation site specificity of neuronal Cdc2- like kinase

AU - Qi, Zhong

AU - Zhu, Xiaodong

AU - Goedert, Michel

AU - Fujita, Donald J.

AU - Wang, Jerry H.

PY - 1998/2/20

Y1 - 1998/2/20

N2 - Neuronal Cdc2-like kinase (Nclk) can be stimulated by heparin in a substrate-dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9-18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.

AB - Neuronal Cdc2-like kinase (Nclk) can be stimulated by heparin in a substrate-dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9-18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.

KW - Alzheimer's disease

KW - Heparin

KW - Neuronal Cdc2-like kinase

KW - Tau

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000072282000022

UR - https://openalex.org/W2029129837

UR - https://www.scopus.com/pages/publications/0032548778

U2 - 10.1016/S0014-5793(98)00096-9

DO - 10.1016/S0014-5793(98)00096-9

M3 - Journal Article

SN - 0014-5793

VL - 423

SP - 227

EP - 230

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Effect of heparin on phosphorylation site specificity of neuronal Cdc2- like kinase

Abstract

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