Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem

Yick Chuen Chan, Yi Kuen Lee, Man Wong, Yitshak Zohar

Research output: Chapter in Book/Conference Proceeding/ReportConference Paper published in a bookpeer-review

1 Citation (Scopus)

Abstract

This paper presents an experimental and numerical study of the effect of a sub-micron pillar array, embedded in the separation channel, on DNA kinetics in a free-solution capillary electrophoresis microsystem. DNA plugs have been observed to migrate with different velocity depending on the cross-sectional shape of the pillars. This velocity variation can be partly explained by the electric field re-distribution due to the pillar array. Numerical simulations have been carried out to verify the effect of the sub-micron pillar geometry on the electric field distribution, and the computations of DNA plug velocity are qualitatively consistent with the measurements.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages337-339
Number of pages3
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 9 Oct 200513 Oct 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Conference

Conference9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period9/10/0513/10/05

Keywords

  • DNA kinetics
  • Electrophoresis
  • Free-solution
  • Sub-micron pillars

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