Estimation of the length of cell cycle phases from asynchronous cultures of Saccharomyces cerevisiae

J. P. Barford; R. J. Hall

doi:10.1016/0014-4827(76)90043-4

Estimation of the length of cell cycle phases from asynchronous cultures of Saccharomyces cerevisiae

J. P. Barford^*, R. J. Hall

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

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Abstract

A method is described which allows estimation of the lengths of the cell cycle phases of Saccharomyces cerevisiae from asynchronous culture data. It is based upon a DNA labelling and nuclear staining procedure which is related to known features of yeast physiology. Estimates obtained indicate that the lengths of the periods of DNA synthesis (S phase) and mitosis (M phase) are relatively independent of the carbon source on which the culture was grown, whilst the lengths of the G1 and G2 phases show considerable variation.

Original language	English
Pages (from-to)	276-284
Number of pages	9
Journal	Experimental Cell Research
Volume	102
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(76)90043-4
Publication status	Published - 15 Oct 1976
Externally published	Yes

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10.1016/0014-4827(76)90043-4

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title = "Estimation of the length of cell cycle phases from asynchronous cultures of Saccharomyces cerevisiae",

abstract = "A method is described which allows estimation of the lengths of the cell cycle phases of Saccharomyces cerevisiae from asynchronous culture data. It is based upon a DNA labelling and nuclear staining procedure which is related to known features of yeast physiology. Estimates obtained indicate that the lengths of the periods of DNA synthesis (S phase) and mitosis (M phase) are relatively independent of the carbon source on which the culture was grown, whilst the lengths of the G1 and G2 phases show considerable variation.",

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AU - Barford, J. P.

AU - Hall, R. J.

PY - 1976/10/15

Y1 - 1976/10/15

N2 - A method is described which allows estimation of the lengths of the cell cycle phases of Saccharomyces cerevisiae from asynchronous culture data. It is based upon a DNA labelling and nuclear staining procedure which is related to known features of yeast physiology. Estimates obtained indicate that the lengths of the periods of DNA synthesis (S phase) and mitosis (M phase) are relatively independent of the carbon source on which the culture was grown, whilst the lengths of the G1 and G2 phases show considerable variation.

AB - A method is described which allows estimation of the lengths of the cell cycle phases of Saccharomyces cerevisiae from asynchronous culture data. It is based upon a DNA labelling and nuclear staining procedure which is related to known features of yeast physiology. Estimates obtained indicate that the lengths of the periods of DNA synthesis (S phase) and mitosis (M phase) are relatively independent of the carbon source on which the culture was grown, whilst the lengths of the G1 and G2 phases show considerable variation.

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UR - https://www.scopus.com/pages/publications/0017186833

U2 - 10.1016/0014-4827(76)90043-4

DO - 10.1016/0014-4827(76)90043-4

M3 - Journal Article

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VL - 102

SP - 276

EP - 284

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Estimation of the length of cell cycle phases from asynchronous cultures of Saccharomyces cerevisiae

Abstract

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