Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique

C. P. Marquis; J. P. Barford; C. Harbour; D. M. Nobbs

doi:10.1007/BF00153748

Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique

C. P. Marquis^*, J. P. Barford, C. Harbour, D. M. Nobbs

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

2 Citations (Scopus)

Abstract

The analysis of free amino acids in mammalian cell culture media can give valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potential limiting nutrients. However, analysis over the whole culture period enables a kinetic approach to allow understanding of amino acid uptake and potential strategies to improve media design. This communication details the use of a less commonly used HPLC protocol, indicating various advantages and disadvantages. Further, the batch kinetics of amino acid metabolism of two cell lines are discussed.

Original language	English
Pages (from-to)	799-804
Number of pages	6
Journal	Biotechnology techniques
Volume	7
Issue number	11
DOIs	https://doi.org/10.1007/BF00153748
Publication status	Published - Oct 1993
Externally published	Yes

Keywords

amino acid
hplc
mammalian cell culture

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10.1007/BF00153748

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title = "Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique",

abstract = "The analysis of free amino acids in mammalian cell culture media can give valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potential limiting nutrients. However, analysis over the whole culture period enables a kinetic approach to allow understanding of amino acid uptake and potential strategies to improve media design. This communication details the use of a less commonly used HPLC protocol, indicating various advantages and disadvantages. Further, the batch kinetics of amino acid metabolism of two cell lines are discussed.",

keywords = "amino acid, hplc, mammalian cell culture",

author = "Marquis, \{C. P.\} and Barford, \{J. P.\} and C. Harbour and Nobbs, \{D. M.\}",

year = "1993",

month = oct,

doi = "10.1007/BF00153748",

language = "English",

volume = "7",

pages = "799--804",

journal = "Biotechnology techniques",

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publisher = "Kluwer Academic Publishers",

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Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique. / Marquis, C. P.; Barford, J. P.; Harbour, C. et al.
In: Biotechnology techniques, Vol. 7, No. 11, 10.1993, p. 799-804.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique

AU - Marquis, C. P.

AU - Barford, J. P.

AU - Harbour, C.

AU - Nobbs, D. M.

PY - 1993/10

Y1 - 1993/10

N2 - The analysis of free amino acids in mammalian cell culture media can give valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potential limiting nutrients. However, analysis over the whole culture period enables a kinetic approach to allow understanding of amino acid uptake and potential strategies to improve media design. This communication details the use of a less commonly used HPLC protocol, indicating various advantages and disadvantages. Further, the batch kinetics of amino acid metabolism of two cell lines are discussed.

AB - The analysis of free amino acids in mammalian cell culture media can give valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potential limiting nutrients. However, analysis over the whole culture period enables a kinetic approach to allow understanding of amino acid uptake and potential strategies to improve media design. This communication details the use of a less commonly used HPLC protocol, indicating various advantages and disadvantages. Further, the batch kinetics of amino acid metabolism of two cell lines are discussed.

KW - amino acid

KW - hplc

KW - mammalian cell culture

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U2 - 10.1007/BF00153748

DO - 10.1007/BF00153748

M3 - Journal Article

SN - 0951-208X

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JO - Biotechnology techniques

JF - Biotechnology techniques

IS - 11

ER -

Evaluation of the batch kinetics of the amino acid metabolism of a mouse hybridoma and human lymphoblastoid cell line using a pre-column FDNDEA derivitisation, HPLC technique

Abstract

Keywords

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