TY - JOUR
T1 - HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways
AU - Lv, Daojun
AU - Wu, Huayan
AU - Xing, Rongwei
AU - Shu, Fangpeng
AU - Lei, Bin
AU - Lei, Chengyong
AU - Zhou, Xumin
AU - Wan, Bo
AU - Yang, Yu
AU - Zhong, Liren
AU - Mao, Xiangming
AU - Zou, Yaguang
PY - 2017
Y1 - 2017
N2 - Heterogeneous nuclear ribonucleoprotein L (hnRNP-L) is a promoter of various kinds of cancers, but its actions in bladder cancer (BC) are unclear. In this study, we investigated the function and the underlying mechanism of hnRNP-L in bladder carcinogenesis. Our results demonstrated that enhanced hnRNP-L expression in BC tissues was associated with poor overall survival of BC patients. Depletion of hnRNP-L significantly suppressed cell proliferation in vitro and inhibited xenograft tumor growth in vivo. Furthermore, downregulation of hnRNP-L resulted in G1-phase cell cycle arrest and enhanced apoptosis accompanied by inhibition of EMT and cell migration. All these cellular changes were reversed by ectopic expression of hnRNP-L. Deletion of hnRNP-L resulted in decreased expression of Bcl-2, enhanced expression of caspases-3, -6 and -9 and inhibition of the MAPK signaling pathway. These findings demonstrate that hnRNP-L contributes to poor prognosis and tumor progression of BC by inhibiting the intrinsic apoptotic signaling and enhancing MAPK signaling pathways.
AB - Heterogeneous nuclear ribonucleoprotein L (hnRNP-L) is a promoter of various kinds of cancers, but its actions in bladder cancer (BC) are unclear. In this study, we investigated the function and the underlying mechanism of hnRNP-L in bladder carcinogenesis. Our results demonstrated that enhanced hnRNP-L expression in BC tissues was associated with poor overall survival of BC patients. Depletion of hnRNP-L significantly suppressed cell proliferation in vitro and inhibited xenograft tumor growth in vivo. Furthermore, downregulation of hnRNP-L resulted in G1-phase cell cycle arrest and enhanced apoptosis accompanied by inhibition of EMT and cell migration. All these cellular changes were reversed by ectopic expression of hnRNP-L. Deletion of hnRNP-L resulted in decreased expression of Bcl-2, enhanced expression of caspases-3, -6 and -9 and inhibition of the MAPK signaling pathway. These findings demonstrate that hnRNP-L contributes to poor prognosis and tumor progression of BC by inhibiting the intrinsic apoptotic signaling and enhancing MAPK signaling pathways.
KW - Bladder cancer
KW - Epithelial-mesenchymal transition
KW - Heterogeneous nuclear ribonucleoprotein L
KW - Intrinsic apoptosis
KW - MAPK
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000395692000099
UR - https://openalex.org/W2575357329
UR - https://www.scopus.com/pages/publications/85013392726
U2 - 10.18632/oncotarget.14600
DO - 10.18632/oncotarget.14600
M3 - Journal Article
SN - 1949-2553
VL - 8
SP - 13586
EP - 13599
JO - Oncotarget
JF - Oncotarget
IS - 8
ER -
