Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array

Yuxin Hu; Zhengke Wang; Yonghong Wang; Fang Bao; Ning Li; Zhenhua Peng; Jiayang Li

Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array

Yuxin Hu, Zhengke Wang, Yonghong Wang, Fang Bao, Ning Li, Zhenhua Peng, Jiayang Li^*

^*Corresponding author for this work

Division of Life Science

Research output: Contribution to journal › Journal Article › peer-review

14 Citations (Scopus)

Abstract

We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutant de12 suspension culture of Arabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designated BRR1-BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, sucn as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.

Original language	English
Pages (from-to)	637-643
Number of pages	7
Journal	Science in China, Series C: Life Sciences
Volume	44
Issue number	6
Publication status	Published - 2001

Keywords

Arabidopsis thaliana
Brassinosteroid responsive gene (BRR)
cDNA array

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title = "Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array",

abstract = "We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutant de12 suspension culture of Arabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designated BRR1-BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, sucn as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.",

keywords = "Arabidopsis thaliana, Brassinosteroid responsive gene (BRR), cDNA array",

author = "Yuxin Hu and Zhengke Wang and Yonghong Wang and Fang Bao and Ning Li and Zhenhua Peng and Jiayang Li",

year = "2001",

language = "English",

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pages = "637--643",

journal = "Science in China, Series C: Life Sciences",

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TY - JOUR

T1 - Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array

AU - Hu, Yuxin

AU - Wang, Zhengke

AU - Wang, Yonghong

AU - Bao, Fang

AU - Li, Ning

AU - Peng, Zhenhua

AU - Li, Jiayang

PY - 2001

Y1 - 2001

N2 - We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutant de12 suspension culture of Arabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designated BRR1-BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, sucn as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.

AB - We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutant de12 suspension culture of Arabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designated BRR1-BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, sucn as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.

KW - Arabidopsis thaliana

KW - Brassinosteroid responsive gene (BRR)

KW - cDNA array

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000175029600010

UR - https://www.scopus.com/pages/publications/33744835554

M3 - Journal Article

SN - 1006-9305

VL - 44

SP - 637

EP - 643

JO - Science in China, Series C: Life Sciences

JF - Science in China, Series C: Life Sciences

IS - 6

ER -

Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array

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Keywords

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