Laser raman spectroscopy of the Lens in situ, measured in an anesthetized rabbit

Nai Teng Yu; John F.R. Kuck; Carl C. Askren

doi:10.3109/02713688109001863

Laser raman spectroscopy of the Lens in situ, measured in an anesthetized rabbit

Nai Teng Yu^*, John F.R. Kuck, Carl C. Askren

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

25 Citations (Scopus)

Abstract

We have obtained the first Raman spectrum from the lens of a live animal. A laser beam (514.5 nm; 15 mW) was directed into the eye of an anesthetized rabbit at 60° from the visual axis and Raman emission was collected at 90° from the incident beam. The power density at the retina was estimated at 0.5 W/cm². The entire scattering column in the lens can be imaged on the entrance slit of a spectrometer with so little distortion that Raman "optical dissection" analysis (Askren, Yu and Kuck (1979) Exp. Eye Res. 29_, 647) can be performed on the in situ lens. The advantages of multichannel detectors over photomultiplier tubes with respect to in situ measurements are discussed.

Original language	English
Pages (from-to)	615-618
Number of pages	4
Journal	Current Eye Research
Volume	1
Issue number	10
DOIs	https://doi.org/10.3109/02713688109001863
Publication status	Published - 1981
Externally published	Yes

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title = "Laser raman spectroscopy of the Lens in situ, measured in an anesthetized rabbit",

abstract = "We have obtained the first Raman spectrum from the lens of a live animal. A laser beam (514.5 nm; 15 mW) was directed into the eye of an anesthetized rabbit at 60° from the visual axis and Raman emission was collected at 90° from the incident beam. The power density at the retina was estimated at 0.5 W/cm2. The entire scattering column in the lens can be imaged on the entrance slit of a spectrometer with so little distortion that Raman {"}optical dissection{"} analysis (Askren, Yu and Kuck (1979) Exp. Eye Res. 29\_, 647) can be performed on the in situ lens. The advantages of multichannel detectors over photomultiplier tubes with respect to in situ measurements are discussed.",

author = "Yu, \{Nai Teng\} and Kuck, \{John F.R.\} and Askren, \{Carl C.\}",

year = "1981",

doi = "10.3109/02713688109001863",

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T1 - Laser raman spectroscopy of the Lens in situ, measured in an anesthetized rabbit

AU - Yu, Nai Teng

AU - Kuck, John F.R.

AU - Askren, Carl C.

PY - 1981

Y1 - 1981

N2 - We have obtained the first Raman spectrum from the lens of a live animal. A laser beam (514.5 nm; 15 mW) was directed into the eye of an anesthetized rabbit at 60° from the visual axis and Raman emission was collected at 90° from the incident beam. The power density at the retina was estimated at 0.5 W/cm2. The entire scattering column in the lens can be imaged on the entrance slit of a spectrometer with so little distortion that Raman "optical dissection" analysis (Askren, Yu and Kuck (1979) Exp. Eye Res. 29_, 647) can be performed on the in situ lens. The advantages of multichannel detectors over photomultiplier tubes with respect to in situ measurements are discussed.

AB - We have obtained the first Raman spectrum from the lens of a live animal. A laser beam (514.5 nm; 15 mW) was directed into the eye of an anesthetized rabbit at 60° from the visual axis and Raman emission was collected at 90° from the incident beam. The power density at the retina was estimated at 0.5 W/cm2. The entire scattering column in the lens can be imaged on the entrance slit of a spectrometer with so little distortion that Raman "optical dissection" analysis (Askren, Yu and Kuck (1979) Exp. Eye Res. 29_, 647) can be performed on the in situ lens. The advantages of multichannel detectors over photomultiplier tubes with respect to in situ measurements are discussed.

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VL - 1

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EP - 618

JO - Current Eye Research

JF - Current Eye Research

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ER -

Laser raman spectroscopy of the Lens in situ, measured in an anesthetized rabbit

Abstract

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