Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

Stijn Vandewiele; Filip Strubbe; Caspar Schreuer; Kristiaan Neyts; Filip Beunis

doi:10.1364/OE.25.025853

Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

Stijn Vandewiele^*, Filip Strubbe, Caspar Schreuer, Kristiaan Neyts, Filip Beunis

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

12 Citations (Scopus)

Abstract

We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4% for the index of refraction and 2.2% for the diameter of the particles.

Original language	English
Pages (from-to)	25853-25866
Number of pages	14
Journal	Optics Express
Volume	25
Issue number	21
DOIs	https://doi.org/10.1364/OE.25.025853
Publication status	Published - 16 Oct 2017
Externally published	Yes

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© 2017 Optical Society of America.

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abstract = "We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4\% for the index of refraction and 2.2\% for the diameter of the particles.",

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AU - Vandewiele, Stijn

AU - Strubbe, Filip

AU - Schreuer, Caspar

AU - Neyts, Kristiaan

AU - Beunis, Filip

PY - 2017/10/16

Y1 - 2017/10/16

N2 - We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4% for the index of refraction and 2.2% for the diameter of the particles.

AB - We demonstrate the use of low spatial and temporal coherence holography microscopy, based on the Lorenz-Mie model, using the standard tungsten-halogen lamp present in an inverted microscope. An optical model is put forward to incorporate the effect of spectral width and different incidence angles of the incident light determined by the aperture at the back focal plane of the condenser lens. The model is validated for 899 nm diameter polystyrene microspheres in glycerol, giving a resolution of 0.4% for the index of refraction and 2.2% for the diameter of the particles.

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Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

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