Abstract
In this work, we show the fabrication, characterization and application of miniaturized free-flow isoelectric focusing platforms containing a covalently immobilized aminoperylene bisimide-based near infrared (NIR) fluorescent pH sensing layer obtained via photocopolymerization with acryloylmorpholine and oligoethyleneglycol diacrylate. These platforms were obtained via multistep photopolymerization and enable process observation as well as analyte identification via their isoelectric points during isoelectric focusing. These microchips were characterized and used for continuous separations of Atto 425 labeled proteins with in-line quantification of their isoelectric points.
| Original language | English |
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| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2155-2157 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 |
| Externally published | Yes |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- In-line analysis
- Isoelectric point quantification
- Micro free-flow electrophoresis
- Microchip isoelectric focusing
- NIR fluorescent pH sensor