Microfluidic flow reactors with integrated micro-heaters and fluorescent temperature sensors for reaction monitoring

Christian Höra, Zhe Shu, Erik Beckert, Stefan Nagl, Detlev Belder*

*Corresponding author for this work

Research output: Chapter in Book/Conference Proceeding/ReportConference Paper published in a bookpeer-review

Abstract

We present the combined integration of fluorescent temperature sensor layers and resistive micro-heaters in microfluidic reactor chips and their application for temperature control and monitoring during an on-chip enzymatic conversion. The production process of the chips consists of wet chemical etching, photolithography, blade coating and soft lithography. These multifunctional chips allow both temperature control and microscopic imaging of the local temperature in microfluidic flow reactors. They are thus able to determine the temperature dependency of chemical and biological reaction on the microscale. This is demonstrated by the tryptic cleavage of modified peptides yielding a fluorescent product at different temperatures.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages784-786
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Enzymatic reaction
  • Flow reactor
  • Fluorescent temperature sensor
  • Microheater
  • Microscopic temperature control

Fingerprint

Dive into the research topics of 'Microfluidic flow reactors with integrated micro-heaters and fluorescent temperature sensors for reaction monitoring'. Together they form a unique fingerprint.

Cite this