Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos

Sarah E. Webb; Andrew L. Miller

doi:10.1101/pdb.prot072967

Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos

Sarah E. Webb
, Andrew L. Miller

Division of Life Science

Research output: Contribution to journal › Journal Article › peer-review

10 Citations (Scopus)

Abstract

The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca²⁺ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.

Original language	English
Pages (from-to)	447-455
Number of pages	9
Journal	Cold Spring Harbor Protocols
Volume	8
Issue number	5
DOIs	https://doi.org/10.1101/pdb.prot072967
Publication status	Published - May 2013

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10.1101/pdb.prot072967

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title = "Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos",

abstract = "The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca2+ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.",

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AU - Webb, Sarah E.

AU - Miller, Andrew L.

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N2 - The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca2+ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.

AB - The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca2+ to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.

UR - https://openalex.org/W2321984123

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DO - 10.1101/pdb.prot072967

M3 - Journal Article

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VL - 8

SP - 447

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JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 5

ER -

Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos

Abstract

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