TY - UNPB
T1 - Mode of Action of Elasnin as Biofilm-Formation Eradicator of Methicillin-Resistant Staphylococcus Aureus
AU - Sulaiman, Jordy Evan
AU - Chen, Feng
AU - Cheng, Aifang
AU - Lam, Hei Ning
AU - Li, Yongxin
AU - Liu, Wenchao
AU - Long, Lexin
AU - Malit, Jessie James Limlingan
AU - Qian, Peiyuan
AU - Wang, Ruojun
AU - Wong, Wai Chuen
AU - Xiao, Yao
PY - 2021/8
Y1 - 2021/8
N2 - Biofilm is made by microbes and often offers protection by making them more tolerant, resistant, and resilient to wide-range antimicrobials. Biofilm-related infections account for more than 80% of human bacterial infections and are especially prevalent in chronic tissue and device-related infections. Owing to the great challenge in treating biofilms, novel and effective antibiofilm compounds urgently need to be identified. We herein identified elasnin as a potent biofilm-targeting compound against methicillin-resistant Staphylococcus aureus (MRSA) through bioassay-guided isolation of bioactive compounds from Actinobacteria. Elasnin effectively inhibited biofilm formation and eradicated pre-formed biofilms of MRSA, and it displays low cytotoxicity with a low risk of resistance development. Transcriptomic and proteomic analyses combined with confocal microscopy observation revealed that elasnin destroyed the biofilm matrix in a time-dependent manner and interfered with cell cycle during the exponential phase, primarily by repressing the expression of virulence factors. Moreover, the biofilm cells released from elasnin treatment showed increased sensitivity to penicillin G. Overall, this study identified elasnin as a promising biofilm-eradicating compound against MRSA and shed light on its action mechanism.
AB - Biofilm is made by microbes and often offers protection by making them more tolerant, resistant, and resilient to wide-range antimicrobials. Biofilm-related infections account for more than 80% of human bacterial infections and are especially prevalent in chronic tissue and device-related infections. Owing to the great challenge in treating biofilms, novel and effective antibiofilm compounds urgently need to be identified. We herein identified elasnin as a potent biofilm-targeting compound against methicillin-resistant Staphylococcus aureus (MRSA) through bioassay-guided isolation of bioactive compounds from Actinobacteria. Elasnin effectively inhibited biofilm formation and eradicated pre-formed biofilms of MRSA, and it displays low cytotoxicity with a low risk of resistance development. Transcriptomic and proteomic analyses combined with confocal microscopy observation revealed that elasnin destroyed the biofilm matrix in a time-dependent manner and interfered with cell cycle during the exponential phase, primarily by repressing the expression of virulence factors. Moreover, the biofilm cells released from elasnin treatment showed increased sensitivity to penicillin G. Overall, this study identified elasnin as a promising biofilm-eradicating compound against MRSA and shed light on its action mechanism.
UR - https://openalex.org/W3194548591
U2 - 10.21203/rs.3.rs-752510/v1
DO - 10.21203/rs.3.rs-752510/v1
M3 - Preprint
T3 - Research Square
BT - Mode of Action of Elasnin as Biofilm-Formation Eradicator of Methicillin-Resistant Staphylococcus Aureus
PB - Frontiers Media SA
ER -