TY - GEN
T1 - Nano knife fabrication and calibration for single cell cutting inside environmental SEM
AU - Shen, Yajing
AU - Nakajima, Masahiro
AU - Kojima, Seiji
AU - Homma, Michio
AU - Fukuda, Toshio
PY - 2010
Y1 - 2010
N2 - The current techniques for cell cutting always use a diamond knife with an edge angle larger than 20°. The compression caused by the edge angle may destroy the sample. In this paper, a nano knife with a small edge angle 5° was fabricated from a commercial AFM cantilever by FIB etching. The mechanical character of the nano knife was calibrated by theoretical calculation, numerical simulation and nanorobotic manipulation methods respectively. Single cell cutting was performed by using the nanorobotic manipulation system inside an Environment-SEM. The maximum cutting force was calculated based on the deformation of the nano knife beam. The calibration and experimental result showed the nano knife was capable for cell cutting task. The angle between two cell slices after cutting was around 15°, which means the compression force caused by the knife edge angle can be reduced.
AB - The current techniques for cell cutting always use a diamond knife with an edge angle larger than 20°. The compression caused by the edge angle may destroy the sample. In this paper, a nano knife with a small edge angle 5° was fabricated from a commercial AFM cantilever by FIB etching. The mechanical character of the nano knife was calibrated by theoretical calculation, numerical simulation and nanorobotic manipulation methods respectively. Single cell cutting was performed by using the nanorobotic manipulation system inside an Environment-SEM. The maximum cutting force was calculated based on the deformation of the nano knife beam. The calibration and experimental result showed the nano knife was capable for cell cutting task. The angle between two cell slices after cutting was around 15°, which means the compression force caused by the knife edge angle can be reduced.
UR - https://openalex.org/W1992710723
UR - https://www.scopus.com/pages/publications/78751479358
U2 - 10.1109/MHS.2010.5669527
DO - 10.1109/MHS.2010.5669527
M3 - Conference Paper published in a book
SN - 9781424479962
T3 - 2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
SP - 316
EP - 320
BT - 2010 International Symposium on Micro-NanoMechatronics and Human Science
T2 - 21st Annual Symposium on Micro-Nano Mechatronics and Human Science, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010
Y2 - 7 November 2010 through 10 November 2010
ER -