Nicotiana chloroplast genome - IV. N. accuminata has larger inverted repeats and genome size

G. F. Shen; K. Chen; M. Wu; S. D. Kung

doi:10.1007/BF00384377

Nicotiana chloroplast genome - IV. N. accuminata has larger inverted repeats and genome size

G. F. Shen^*, K. Chen, M. Wu, S. D. Kung

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

27 Citations (Scopus)

Abstract

N. accuminata has a chloroplast genome of 171 Kb which is larger than 160 Kb reported for N. tabacum. A physical map of the former has been constructed by SalI, BglI, and PvuII enzymes in comparison with N. tabacum. They both share identical restriction map except in the extra segment of N. accuminata. This extra segment is located on the right-hand border of an inverted repeat in the large-single copy region of N. accuminata. It contains the following restriction sites: two for BglI and SmaI; three for SalI and XhoI. The size of inverted repeat measured by electron microscope is 22.67±0.78 Kb and 19.28±0.61 Kb for N. accuminata and N. tabacum, respectively. This is the first case where a larger inverted repeat region accompanied by a larger genome size was found among over 30 Nicotiana species studied thus far.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	MOLECULAR AND GENERAL GENETICS
Volume	187
Issue number	1
DOIs	https://doi.org/10.1007/BF00384377
Publication status	Published - Sept 1982
Externally published	Yes

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title = "Nicotiana chloroplast genome - IV. N. accuminata has larger inverted repeats and genome size",

abstract = "N. accuminata has a chloroplast genome of 171 Kb which is larger than 160 Kb reported for N. tabacum. A physical map of the former has been constructed by SalI, BglI, and PvuII enzymes in comparison with N. tabacum. They both share identical restriction map except in the extra segment of N. accuminata. This extra segment is located on the right-hand border of an inverted repeat in the large-single copy region of N. accuminata. It contains the following restriction sites: two for BglI and SmaI; three for SalI and XhoI. The size of inverted repeat measured by electron microscope is 22.67±0.78 Kb and 19.28±0.61 Kb for N. accuminata and N. tabacum, respectively. This is the first case where a larger inverted repeat region accompanied by a larger genome size was found among over 30 Nicotiana species studied thus far.",

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T1 - Nicotiana chloroplast genome - IV. N. accuminata has larger inverted repeats and genome size

AU - Shen, G. F.

AU - Chen, K.

AU - Wu, M.

AU - Kung, S. D.

PY - 1982/9

Y1 - 1982/9

N2 - N. accuminata has a chloroplast genome of 171 Kb which is larger than 160 Kb reported for N. tabacum. A physical map of the former has been constructed by SalI, BglI, and PvuII enzymes in comparison with N. tabacum. They both share identical restriction map except in the extra segment of N. accuminata. This extra segment is located on the right-hand border of an inverted repeat in the large-single copy region of N. accuminata. It contains the following restriction sites: two for BglI and SmaI; three for SalI and XhoI. The size of inverted repeat measured by electron microscope is 22.67±0.78 Kb and 19.28±0.61 Kb for N. accuminata and N. tabacum, respectively. This is the first case where a larger inverted repeat region accompanied by a larger genome size was found among over 30 Nicotiana species studied thus far.

AB - N. accuminata has a chloroplast genome of 171 Kb which is larger than 160 Kb reported for N. tabacum. A physical map of the former has been constructed by SalI, BglI, and PvuII enzymes in comparison with N. tabacum. They both share identical restriction map except in the extra segment of N. accuminata. This extra segment is located on the right-hand border of an inverted repeat in the large-single copy region of N. accuminata. It contains the following restriction sites: two for BglI and SmaI; three for SalI and XhoI. The size of inverted repeat measured by electron microscope is 22.67±0.78 Kb and 19.28±0.61 Kb for N. accuminata and N. tabacum, respectively. This is the first case where a larger inverted repeat region accompanied by a larger genome size was found among over 30 Nicotiana species studied thus far.

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DO - 10.1007/BF00384377

M3 - Journal Article

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SN - 0026-8925

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JO - MOLECULAR AND GENERAL GENETICS

JF - MOLECULAR AND GENERAL GENETICS

IS - 1

ER -

Nicotiana chloroplast genome - IV. N. accuminata has larger inverted repeats and genome size

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