Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes

Frank Wendzinski; Bernd Gruendig; Reinhard Renneberg; Karl Cammann; Friedrich Spener

Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes

Frank Wendzinski^*, Bernd Gruendig, Reinhard Renneberg, Karl Cammann, Friedrich Spener

^*Corresponding author for this work

Research output: Contribution to conference › Conference Paper › peer-review

Abstract

Redox mediators enable an efficient electron transfer between redox-enzymes and the electrochemical surface of amperometric sensors. A stable signal was obtained using a tetrathiafulvalene (TTF) modified carbon electrode. In the presence of horseradish peroxidase (HRP) hydrogen peroxide could be monitored with a detection limit of 7 nM at a potential of + 20 mV vs SCE. With a constant concentration of hydrogen peroxide the detection limit for HRP was 7 ng/ml. Using TTF-modified carbon electrodes, rabbit immunoglobulin could be detected in a sandwich approach with a range between 5 and 100 ng/ml. In flow-through systems the preference of consecutive measurements of HRP could be increased by a short polarization of the TTF-modified carbon electrode at + 100 mV.

Original language	English
Pages	535-537
Number of pages	3
Publication status	Published - 1995
Externally published	Yes
Event	Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2) - Stockholm, Sweden Duration: 25 Jun 1995 → 29 Jun 1995

Conference

Conference	Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2)
City	Stockholm, Sweden
Period	25/06/95 → 29/06/95

Cite this

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title = "Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes",

abstract = "Redox mediators enable an efficient electron transfer between redox-enzymes and the electrochemical surface of amperometric sensors. A stable signal was obtained using a tetrathiafulvalene (TTF) modified carbon electrode. In the presence of horseradish peroxidase (HRP) hydrogen peroxide could be monitored with a detection limit of 7 nM at a potential of + 20 mV vs SCE. With a constant concentration of hydrogen peroxide the detection limit for HRP was 7 ng/ml. Using TTF-modified carbon electrodes, rabbit immunoglobulin could be detected in a sandwich approach with a range between 5 and 100 ng/ml. In flow-through systems the preference of consecutive measurements of HRP could be increased by a short polarization of the TTF-modified carbon electrode at + 100 mV.",

author = "Frank Wendzinski and Bernd Gruendig and Reinhard Renneberg and Karl Cammann and Friedrich Spener",

year = "1995",

language = "English",

pages = "535--537",

note = "Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2) ; Conference date: 25-06-1995 Through 29-06-1995",

}

Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes. / Wendzinski, Frank; Gruendig, Bernd; Renneberg, Reinhard et al.
1995. 535-537 Paper presented at Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2), Stockholm, Sweden.

Research output: Contribution to conference › Conference Paper › peer-review

TY - CONF

T1 - Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes

AU - Wendzinski, Frank

AU - Gruendig, Bernd

AU - Renneberg, Reinhard

AU - Cammann, Karl

AU - Spener, Friedrich

PY - 1995

Y1 - 1995

N2 - Redox mediators enable an efficient electron transfer between redox-enzymes and the electrochemical surface of amperometric sensors. A stable signal was obtained using a tetrathiafulvalene (TTF) modified carbon electrode. In the presence of horseradish peroxidase (HRP) hydrogen peroxide could be monitored with a detection limit of 7 nM at a potential of + 20 mV vs SCE. With a constant concentration of hydrogen peroxide the detection limit for HRP was 7 ng/ml. Using TTF-modified carbon electrodes, rabbit immunoglobulin could be detected in a sandwich approach with a range between 5 and 100 ng/ml. In flow-through systems the preference of consecutive measurements of HRP could be increased by a short polarization of the TTF-modified carbon electrode at + 100 mV.

AB - Redox mediators enable an efficient electron transfer between redox-enzymes and the electrochemical surface of amperometric sensors. A stable signal was obtained using a tetrathiafulvalene (TTF) modified carbon electrode. In the presence of horseradish peroxidase (HRP) hydrogen peroxide could be monitored with a detection limit of 7 nM at a potential of + 20 mV vs SCE. With a constant concentration of hydrogen peroxide the detection limit for HRP was 7 ng/ml. Using TTF-modified carbon electrodes, rabbit immunoglobulin could be detected in a sandwich approach with a range between 5 and 100 ng/ml. In flow-through systems the preference of consecutive measurements of HRP could be increased by a short polarization of the TTF-modified carbon electrode at + 100 mV.

UR - https://www.scopus.com/pages/publications/0029489632

M3 - Conference Paper

AN - SCOPUS:0029489632

SP - 535

EP - 537

T2 - Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2)

Y2 - 25 June 1995 through 29 June 1995

ER -

Pseudo-homogeneous immunosensing with tetrathiafulvalene-based electrodes

Abstract

Conference

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