Purification of isoacceptor transfer RNA by affinity coupling to aldehyde-containing matrix

Qi Song Wang; J. Tze Fei Wong

doi:10.1016/0003-2697(83)90184-7

Purification of isoacceptor transfer RNA by affinity coupling to aldehyde-containing matrix

Qi Song Wang^*, J. Tze Fei Wong

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

6 Citations (Scopus)

Abstract

A method has been developed for the affinity coupling of aminoacyl-tRNA to an aldehyde-containing polymer by means of reduction with sodium cyanoborohydride. Sephadex dialdehyde obtained from periodate oxidation of Sephadex G-50, and Enzacryl polyaldehyde obtained from hydrolysis of Enzacryl polyacetal, were found to provide the requisite polymeric matrix. This coupling reaction permitted the rapid purification of isoacceptor tRNAs of Escherichia coli for the α-amino acids glycine, valine, and arginine, as well as the α-imino acid proline.

Original language	English
Pages (from-to)	360-364
Number of pages	5
Journal	Analytical Biochemistry
Volume	131
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90184-7
Publication status	Published - Jun 1983
Externally published	Yes

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10.1016/0003-2697(83)90184-7

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title = "Purification of isoacceptor transfer RNA by affinity coupling to aldehyde-containing matrix",

abstract = "A method has been developed for the affinity coupling of aminoacyl-tRNA to an aldehyde-containing polymer by means of reduction with sodium cyanoborohydride. Sephadex dialdehyde obtained from periodate oxidation of Sephadex G-50, and Enzacryl polyaldehyde obtained from hydrolysis of Enzacryl polyacetal, were found to provide the requisite polymeric matrix. This coupling reaction permitted the rapid purification of isoacceptor tRNAs of Escherichia coli for the α-amino acids glycine, valine, and arginine, as well as the α-imino acid proline.",

author = "Wang, \{Qi Song\} and Wong, \{J. Tze Fei\}",

year = "1983",

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journal = "Analytical Biochemistry",

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AU - Wang, Qi Song

AU - Wong, J. Tze Fei

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N2 - A method has been developed for the affinity coupling of aminoacyl-tRNA to an aldehyde-containing polymer by means of reduction with sodium cyanoborohydride. Sephadex dialdehyde obtained from periodate oxidation of Sephadex G-50, and Enzacryl polyaldehyde obtained from hydrolysis of Enzacryl polyacetal, were found to provide the requisite polymeric matrix. This coupling reaction permitted the rapid purification of isoacceptor tRNAs of Escherichia coli for the α-amino acids glycine, valine, and arginine, as well as the α-imino acid proline.

AB - A method has been developed for the affinity coupling of aminoacyl-tRNA to an aldehyde-containing polymer by means of reduction with sodium cyanoborohydride. Sephadex dialdehyde obtained from periodate oxidation of Sephadex G-50, and Enzacryl polyaldehyde obtained from hydrolysis of Enzacryl polyacetal, were found to provide the requisite polymeric matrix. This coupling reaction permitted the rapid purification of isoacceptor tRNAs of Escherichia coli for the α-amino acids glycine, valine, and arginine, as well as the α-imino acid proline.

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U2 - 10.1016/0003-2697(83)90184-7

DO - 10.1016/0003-2697(83)90184-7

M3 - Journal Article

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AN - SCOPUS:0020771767

SN - 0003-2697

VL - 131

SP - 360

EP - 364

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Purification of isoacceptor transfer RNA by affinity coupling to aldehyde-containing matrix

Abstract

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