Sensing a heart infarction marker with surface plasmon resonance spectroscopy

Ulrich Kunz*, Andreas Katerkamp, Reinhard Renneberg, Friedrich Spener, Karl Cammann

*Corresponding author for this work

Research output: Chapter in Book/Conference Proceeding/ReportConference Paper published in a bookpeer-review

6 Citations (Scopus)

Abstract

In this study a direct immunosensor for heart-type fatty acid binding protein (FABP) based on surface plasmon resonance spectroscopy (SPRS) is presented. FABP can be used as a heart infarction marker in clinical diagnostics. The development of a simple and cheap direct optical sensor device is reported in this paper as well as immobilization procedures and optimization of the measuring conditions. The correct working of the SPRS device is controlled by comparing the signals with theoretical calculated values. Two different immunoassay techniques were optimized for a sensitive FABP-analysis. The competitive immunoassay was superior to the sandwich configuration as it had a lower detection limit (100 ng/ml), needed less antibodies and could be carried out in one step.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsAnnamaria V. Scheggi, Francesco Baldini, Pierre R. Coulet, Otto S. Wolfbeis
Pages40-49
Number of pages10
Publication statusPublished - 1995
Externally publishedYes
EventMedical Sensors II and Fiber Optic Sensors - Lille, Fr
Duration: 9 Sept 19949 Sept 1994

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2331
ISSN (Print)0277-786X

Conference

ConferenceMedical Sensors II and Fiber Optic Sensors
CityLille, Fr
Period9/09/949/09/94

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