TY - GEN
T1 - Single cell adhesion force measurement for viability identification using nanorobotic manipulation system inside ESEM
AU - Shen, Yajing
AU - Nakajima, Masahiro
AU - Kojima, Seiji
AU - Homma, Michio
AU - Fukuda, Toshio
PY - 2011
Y1 - 2011
N2 - Cell viability identification is the first step for single cell analysis. In this paper, a novel cell viability identification method was proposed based on the cell adhesion force measurement. Living and dead yeast cell samples were prepared using the stain method. The micro puller for adhesion force measurement was fabricated from atomic force microscopy (AFM) cantilever by focused ion beam (FIB) etching. The spring constant of the micro puller was calibrated by nano manipulation approach. Cell adhesion force measurement was performed by using the nano robotic manipulation system inside an environmental scanning electron microscopy (ESEM). This system can provide the sample observation and manipulation at nano scale. The adhesion force was calculated according to the deformation of the micro puller. The results showed that the adhesion force of living cells is much larger than that of dead cells. Therefore, cell adhesion force is capable for the cell viability identification task at single cell level.
AB - Cell viability identification is the first step for single cell analysis. In this paper, a novel cell viability identification method was proposed based on the cell adhesion force measurement. Living and dead yeast cell samples were prepared using the stain method. The micro puller for adhesion force measurement was fabricated from atomic force microscopy (AFM) cantilever by focused ion beam (FIB) etching. The spring constant of the micro puller was calibrated by nano manipulation approach. Cell adhesion force measurement was performed by using the nano robotic manipulation system inside an environmental scanning electron microscopy (ESEM). This system can provide the sample observation and manipulation at nano scale. The adhesion force was calculated according to the deformation of the micro puller. The results showed that the adhesion force of living cells is much larger than that of dead cells. Therefore, cell adhesion force is capable for the cell viability identification task at single cell level.
UR - https://openalex.org/W2117371325
UR - https://www.scopus.com/pages/publications/80053304029
U2 - 10.1109/NEMS.2011.6017510
DO - 10.1109/NEMS.2011.6017510
M3 - Conference Paper published in a book
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 944
EP - 947
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -