TY - JOUR
T1 - Slc20a1b is essential for hematopoietic stem/progenitor cell expansion in zebrafish
AU - Chen, Jiakui
AU - Li, Gaofei
AU - Lian, Junwei
AU - Ma, Ning
AU - Huang, Zhibin
AU - Li, Jianchao
AU - Wen, Zilong
AU - Zhang, Wenqing
AU - Zhang, Yiyue
N1 - Publisher Copyright:
© 2021, Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2021/12
Y1 - 2021/12
N2 - Hematopoietic stem and progenitor cells (HSPCs) are able to self-renew and can give rise to all blood lineages throughout their lifetime, yet the mechanisms regulating HSPC development have yet to be discovered. In this study, we characterized a hematopoiesis defective zebrafish mutant line named smu07, which was obtained from our previous forward genetic screening, and found the HSPC expansion deficiency in the mutant. Positional cloning identified that slc20a1b, which encodes a sodium phosphate cotransporter, contributed to the smu07 blood phenotype. Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner. Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.
AB - Hematopoietic stem and progenitor cells (HSPCs) are able to self-renew and can give rise to all blood lineages throughout their lifetime, yet the mechanisms regulating HSPC development have yet to be discovered. In this study, we characterized a hematopoiesis defective zebrafish mutant line named smu07, which was obtained from our previous forward genetic screening, and found the HSPC expansion deficiency in the mutant. Positional cloning identified that slc20a1b, which encodes a sodium phosphate cotransporter, contributed to the smu07 blood phenotype. Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner. Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.
KW - HSPC
KW - slc20a1b
KW - zebrafish
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000631331500001
UR - https://openalex.org/W3138395378
UR - https://www.scopus.com/pages/publications/85102817261
U2 - 10.1007/s11427-020-1878-8
DO - 10.1007/s11427-020-1878-8
M3 - Journal Article
SN - 1674-7305
VL - 64
SP - 2186
EP - 2201
JO - Science China Life Sciences
JF - Science China Life Sciences
IS - 12
ER -