TY - JOUR
T1 - Specific imaging and tracking of mitochondria in live cells by a photostable AIE luminogen
AU - Leung, Chris W.T.
AU - Hong, Yuning
AU - Tang, Ben Zhong
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Tracking the dynamics of mitochondrial morphology has attracted much research interest because of its involvement in early stage apoptosis and degenerative conditions. To follow this process, highly specifi c and photostable fl uorescent probes are in demand. Commercially available mitochondria trackers, however, suffer from poor photostability. To overcome this limitation, we have designed and synthesized a fl uorescent agent, tetraphenylethene-triphenylphosphonium (TPE-TPP), for mitochondrial imaging. Inherent from the mitochondrial-targeting ability of TPP groups and the aggregation-induced emission (AIE) characteristics of the TPE core, TPE-TPP possesses high specifi city to mitochondria, superior photostability, and appreciable tolerance to environmental change, allowing imaging and tracking of the mitochondrial morphological changes in a long period of time.
AB - Tracking the dynamics of mitochondrial morphology has attracted much research interest because of its involvement in early stage apoptosis and degenerative conditions. To follow this process, highly specifi c and photostable fl uorescent probes are in demand. Commercially available mitochondria trackers, however, suffer from poor photostability. To overcome this limitation, we have designed and synthesized a fl uorescent agent, tetraphenylethene-triphenylphosphonium (TPE-TPP), for mitochondrial imaging. Inherent from the mitochondrial-targeting ability of TPP groups and the aggregation-induced emission (AIE) characteristics of the TPE core, TPE-TPP possesses high specifi city to mitochondria, superior photostability, and appreciable tolerance to environmental change, allowing imaging and tracking of the mitochondrial morphological changes in a long period of time.
KW - Aggregation-induced emission
KW - Morphological change
KW - Photostable
KW - Triphenylphosphonium
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000343468200003
UR - https://openalex.org/W2413006546
UR - https://www.scopus.com/pages/publications/84954621289
U2 - 10.1007/978-1-4939-1441-8_2
DO - 10.1007/978-1-4939-1441-8_2
M3 - Journal Article
C2 - 25323496
SN - 1064-3745
VL - 1208
SP - 21
EP - 27
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -