TY - JOUR
T1 - Structure and proposed DNA delivery mechanism of a marine roseophage
AU - Huang, Yang
AU - Sun, Hui
AU - Wei, Shuzhen
AU - Cai, Lanlan
AU - Liu, Liqin
AU - Jiang, Yanan
AU - Xin, Jiabao
AU - Chen, Zhenqin
AU - Que, Yuqiong
AU - Kong, Zhibo
AU - Li, Tingting
AU - Yu, Hai
AU - Zhang, Jun
AU - Gu, Ying
AU - Zheng, Qingbing
AU - Li, Shaowei
AU - Zhang, Rui
AU - Xia, Ningshao
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.
AB - Tailed bacteriophages (order, Caudovirales) account for the majority of all phages. However, the long flexible tail of siphophages hinders comprehensive investigation of the mechanism of viral gene delivery. Here, we report the atomic capsid and in-situ structures of the tail machine of the marine siphophage, vB_DshS-R4C (R4C), which infects Roseobacter. The R4C virion, comprising 12 distinct structural protein components, has a unique five-fold vertex of the icosahedral capsid that allows genome delivery. The specific position and interaction pattern of the tail tube proteins determine the atypical long rigid tail of R4C, and further provide negative charge distribution within the tail tube. A ratchet mechanism assists in DNA transmission, which is initiated by an absorption device that structurally resembles the phage-like particle, RcGTA. Overall, these results provide in-depth knowledge into the intact structure and underlining DNA delivery mechanism for the ecologically important siphophages.
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001009499200007
UR - https://openalex.org/W4381052384
UR - https://www.scopus.com/pages/publications/85161966117
U2 - 10.1038/s41467-023-39220-y
DO - 10.1038/s41467-023-39220-y
M3 - Journal Article
SN - 2041-1723
VL - 14
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3609
ER -