TY - GEN
T1 - Study on the envelope fusion proteins of baculovirus
AU - Zhu, Shan Ying
AU - Pang, Jun Xing
AU - Wang, Wen Bing
AU - Li, Bing
AU - Shen, Wei De
PY - 2008
Y1 - 2008
N2 - GP64 homologues, which are found in Group I NPVs, and F proteins (Fa proteins), which are utilized in Group II NPVs, are required for virion entry into the cell. And Group I NPV also encodes homologue of Fa protein, and was named Fb protein. However, Fb protein dose not appear to mediate cell fusion. To further researching these important viral envelope proteins, the expression of GP64 and Fb proteins in Group I NPVs were studied by Real-time quantitative PCR. The results indicated that Fb is not the evolution redundancy gene but an important function gene. In addition, we transfected the fragments of CbNPV Fa and ie-1 into different insect cell lines, and found that CbNPV Fa and ie-1 could not express in BmN and T.ni High Five cells but could express in Sf-9 and Spli cells.
AB - GP64 homologues, which are found in Group I NPVs, and F proteins (Fa proteins), which are utilized in Group II NPVs, are required for virion entry into the cell. And Group I NPV also encodes homologue of Fa protein, and was named Fb protein. However, Fb protein dose not appear to mediate cell fusion. To further researching these important viral envelope proteins, the expression of GP64 and Fb proteins in Group I NPVs were studied by Real-time quantitative PCR. The results indicated that Fb is not the evolution redundancy gene but an important function gene. In addition, we transfected the fragments of CbNPV Fa and ie-1 into different insect cell lines, and found that CbNPV Fa and ie-1 could not express in BmN and T.ni High Five cells but could express in Sf-9 and Spli cells.
KW - Clanis bilineata tiainglauica nucleopolyhedrovirus
KW - F protein
KW - GFP
KW - GP64
KW - Real-time quantitative PCR
UR - https://www.scopus.com/pages/publications/50949122997
U2 - 10.1109/ICBBE.2008.75
DO - 10.1109/ICBBE.2008.75
M3 - Conference Paper published in a book
AN - SCOPUS:50949122997
SN - 9781424417483
T3 - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
SP - 293
EP - 296
BT - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
PB - IEEE Computer Society
T2 - 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008
Y2 - 16 May 2008 through 18 May 2008
ER -