THERMAL ACTIVATION OF PEROXIDASE FROM TOBACCO LEAF MESOPHYLL CELL WALLS

YUEN‐MIN ‐M CHOY; YUM‐SHING ‐S WONG; KIT‐MAN ‐M LAU; KUNG HYUNG

THERMAL ACTIVATION OF PEROXIDASE FROM TOBACCO LEAF MESOPHYLL CELL WALLS

YUEN‐MIN ‐M CHOY, YUM‐SHING ‐S WONG, KIT‐MAN ‐M LAU, KUNG HYUNG^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

5 Citations (Scopus)

Abstract

Cell wall‐bound peroxidase isolated from tobacco leaf mesophll cell walls was found to consist of two isoenzymes (P₁ and P₂). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70°. P₁ increased to 265% and P₂ to 140% of the activities assayed at 27°; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P₁ showed a carbohydrate content of 26.22% when the monosaccharides were analyzed by gas liquid chromatography; P₂ gave 21.45%.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	International Journal of Peptide and Protein Research
Volume	14
Issue number	1
Publication status	Published - Jul 1979
Externally published	Yes

Keywords

carbohydrate contents of isoperoxidases
isoperoxidases
peroxidases
thermal activation
tobacco leaf isoperoxidases

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https://hdl.handle.net/1783.1/51506

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@article{ac0960b4245444b18cae69e3feccb41f,

title = "THERMAL ACTIVATION OF PEROXIDASE FROM TOBACCO LEAF MESOPHYLL CELL WALLS",

abstract = "Cell wall‐bound peroxidase isolated from tobacco leaf mesophll cell walls was found to consist of two isoenzymes (P1 and P2). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70°. P1 increased to 265\% and P2 to 140\% of the activities assayed at 27°; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P1 showed a carbohydrate content of 26.22\% when the monosaccharides were analyzed by gas liquid chromatography; P2 gave 21.45\%.",

keywords = "carbohydrate contents of isoperoxidases, isoperoxidases, peroxidases, thermal activation, tobacco leaf isoperoxidases",

author = "CHOY, \{YUEN‐MIN ‐M\} and WONG, \{YUM‐SHING ‐S\} and LAU, \{KIT‐MAN ‐M\} and KUNG HYUNG",

year = "1979",

month = jul,

language = "English",

volume = "14",

pages = "1--4",

journal = "International Journal of Peptide and Protein Research",

issn = "0367-8377",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - THERMAL ACTIVATION OF PEROXIDASE FROM TOBACCO LEAF MESOPHYLL CELL WALLS

AU - CHOY, YUEN‐MIN ‐M

AU - WONG, YUM‐SHING ‐S

AU - LAU, KIT‐MAN ‐M

AU - HYUNG, KUNG

PY - 1979/7

Y1 - 1979/7

N2 - Cell wall‐bound peroxidase isolated from tobacco leaf mesophll cell walls was found to consist of two isoenzymes (P1 and P2). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70°. P1 increased to 265% and P2 to 140% of the activities assayed at 27°; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P1 showed a carbohydrate content of 26.22% when the monosaccharides were analyzed by gas liquid chromatography; P2 gave 21.45%.

AB - Cell wall‐bound peroxidase isolated from tobacco leaf mesophll cell walls was found to consist of two isoenzymes (P1 and P2). Each exists in the form of a single subunit with the same molecular weight (35 000) as determined by Sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Both isoenzymes exhibited maximum activities at 70°. P1 increased to 265% and P2 to 140% of the activities assayed at 27°; below this temperature the two isoenzymes had the same specific activity. On hydrolysis, P1 showed a carbohydrate content of 26.22% when the monosaccharides were analyzed by gas liquid chromatography; P2 gave 21.45%.

KW - carbohydrate contents of isoperoxidases

KW - isoperoxidases

KW - peroxidases

KW - thermal activation

KW - tobacco leaf isoperoxidases

UR - https://www.scopus.com/pages/publications/0018498666

M3 - Journal Article

SN - 0367-8377

VL - 14

SP - 1

EP - 4

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

IS - 1

ER -

THERMAL ACTIVATION OF PEROXIDASE FROM TOBACCO LEAF MESOPHYLL CELL WALLS

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