TY - JOUR
T1 - Ultrasonic-induced reversible blood-brain barrier opening
T2 - Safety evaluation into the cellular level
AU - Wang, Mengxin
AU - Guo, Shuyuan
AU - Lin, Bingling
AU - Lv, Tao
AU - Zhang, Zhuxia
AU - Hu, Die
AU - Hu, Azhen
AU - Xu, Bingxuan
AU - Qi, Yulong
AU - Liu, Li
AU - Cheng, Guanxun
AU - Chen, Yun
AU - Zheng, Tingting
N1 - Publisher Copyright:
© 2022 Mengxin Wang et al., published by De Gruyter.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - An important function of the blood-brain barrier (BBB) is to protect the central nervous system and maintain its homeostasis, but it is also a major barrier to the intervention and treatment of neurological diseases. Our study aimed at opening the BBB using a noninvasive method, focused ultrasound, screening for 16 different parameter combinations of frequency, peak voltage (Ppeak) and irradiation time. Comparing the results of hematoxylin-eosin staining, serum oxidative damage factor and TUNEL staining under various conditions, we obtained a parameter combination that did not lead to oxidative stress injury and apoptosis: 0.8 mHz + 900 mVpp + 90 s. It will be used as a safety parameter for BBB opening treatment of Parkinson's disease in our subsequent experiments. In addition, the closing time after the BBB opening was verified in magnetic resonance imaging contrast examination and at the tissue level. It is worth mentioning that, different from previous studies, we focused on damage assessment at cellular and molecular levels.
AB - An important function of the blood-brain barrier (BBB) is to protect the central nervous system and maintain its homeostasis, but it is also a major barrier to the intervention and treatment of neurological diseases. Our study aimed at opening the BBB using a noninvasive method, focused ultrasound, screening for 16 different parameter combinations of frequency, peak voltage (Ppeak) and irradiation time. Comparing the results of hematoxylin-eosin staining, serum oxidative damage factor and TUNEL staining under various conditions, we obtained a parameter combination that did not lead to oxidative stress injury and apoptosis: 0.8 mHz + 900 mVpp + 90 s. It will be used as a safety parameter for BBB opening treatment of Parkinson's disease in our subsequent experiments. In addition, the closing time after the BBB opening was verified in magnetic resonance imaging contrast examination and at the tissue level. It is worth mentioning that, different from previous studies, we focused on damage assessment at cellular and molecular levels.
KW - blood
KW - brain barrier
KW - focused ultrasound
KW - oxidative stress injury
KW - reactive oxygen species
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000889326500001
UR - https://openalex.org/W4312895971
U2 - 10.1515/chem-2022-0173
DO - 10.1515/chem-2022-0173
M3 - Journal Article
SN - 2391-5420
VL - 20
SP - 1327
EP - 1336
JO - Open Chemistry
JF - Open Chemistry
IS - 1
ER -