Ultraviolet Photoacoustic Microscopy for Histopathology

Bingxin Huang, Ivy H.M. Wong, Yan Zhang, Terence T.W. Wong*

*Corresponding author for this work

Research output: Chapter in Book/Conference Proceeding/ReportBook Chapterpeer-review

Abstract

This chapter provides an overview of the use of ultraviolet photoa-coustic microscopy (UV-PAM) in histopathology. After introducing the basics of histopathology, we review the principles of PAM and its application in imaging biological tissues. Then, we discuss the different designs of UV-PAM systems and their respective advantages and limitations in histopathology applications. Finally, we explore the recent advances in deep learning-assisted UV-PAM for virtual staining, which has the potential to revolutionize histopathology by enabling the analysis of tissue samples without the need for traditional staining methods. Overall, this chapter offers a comprehensive overview of the use of UV-PAM in histopathology and highlights its potential to advance the field through novel imaging techniques.

Original languageEnglish
Title of host publicationBiomedical Photoacoustics Technology and Applications
PublisherSpringer Nature
Pages499-532
Number of pages34
ISBN (Electronic)9783031614118
ISBN (Print)9783031614101
DOIs
Publication statusPublished - 1 Jan 2024

Bibliographical note

Publisher Copyright:
© The Editor(s) (ifapplicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.

Keywords

  • Deep learning
  • Histological imaging
  • Multispectral imaging
  • Photoacoustic microscopy
  • Virtual staining

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