Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates

Xiandeng Wu; Marcelo Ganzella; Jinchuan Zhou; Shihan Zhu; Reinhard Jahn; Mingjie Zhang

doi:10.1016/j.molcel.2020.10.029

Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates

Xiandeng Wu
, Marcelo Ganzella
, Jinchuan Zhou
, Shihan Zhu
, Reinhard Jahn
, Mingjie Zhang^*

^*Corresponding author for this work

Division of Life Science

Research output: Contribution to journal › Journal Article › peer-review

99 Citations (Scopus)

Abstract

Each presynaptic bouton contains reserve pool synaptic vesicles (SVs) and a few active zone-tethered SVs. Wu et al. show that SVs coat the surface of protein condensates formed by active zone proteins, such as RIM, RIM-BP, and ELKS, but coacervate with synapsin, forming a multi-phase organization with the docked pool SVs surrounded by the bulk reserve pool SVs as observed in presynaptic boutons.

Original language	English
Pages (from-to)	13-24.e7
Journal	Molecular Cell
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2020.10.029
Publication status	Published - 7 Jan 2021

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

biological condensates
liquid-liquid phase separation
membrane organelles
membraneless condensates
postsynaptic density
presynaptic active zone
synapse formation
synaptic transmission

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10.1016/j.molcel.2020.10.029

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title = "Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates",

abstract = "Each presynaptic bouton contains reserve pool synaptic vesicles (SVs) and a few active zone-tethered SVs. Wu et al. show that SVs coat the surface of protein condensates formed by active zone proteins, such as RIM, RIM-BP, and ELKS, but coacervate with synapsin, forming a multi-phase organization with the docked pool SVs surrounded by the bulk reserve pool SVs as observed in presynaptic boutons.",

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author = "Xiandeng Wu and Marcelo Ganzella and Jinchuan Zhou and Shihan Zhu and Reinhard Jahn and Mingjie Zhang",

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T1 - Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates

AU - Wu, Xiandeng

AU - Ganzella, Marcelo

AU - Zhou, Jinchuan

AU - Zhu, Shihan

AU - Jahn, Reinhard

AU - Zhang, Mingjie

PY - 2021/1/7

Y1 - 2021/1/7

N2 - Each presynaptic bouton contains reserve pool synaptic vesicles (SVs) and a few active zone-tethered SVs. Wu et al. show that SVs coat the surface of protein condensates formed by active zone proteins, such as RIM, RIM-BP, and ELKS, but coacervate with synapsin, forming a multi-phase organization with the docked pool SVs surrounded by the bulk reserve pool SVs as observed in presynaptic boutons.

AB - Each presynaptic bouton contains reserve pool synaptic vesicles (SVs) and a few active zone-tethered SVs. Wu et al. show that SVs coat the surface of protein condensates formed by active zone proteins, such as RIM, RIM-BP, and ELKS, but coacervate with synapsin, forming a multi-phase organization with the docked pool SVs surrounded by the bulk reserve pool SVs as observed in presynaptic boutons.

KW - biological condensates

KW - liquid-liquid phase separation

KW - membrane organelles

KW - membraneless condensates

KW - postsynaptic density

KW - presynaptic active zone

KW - synapse formation

KW - synaptic transmission

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000641169300004

UR - https://openalex.org/W3098847374

U2 - 10.1016/j.molcel.2020.10.029

DO - 10.1016/j.molcel.2020.10.029

M3 - Journal Article

SN - 1097-2765

VL - 81

SP - 13-24.e7

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates

Abstract

Bibliographical note

Keywords

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