Dek modulates global intron retention during muscle stem cells quiescence exit

Abstract

Adult stem cells are essential for tissue regeneration. However, the mechanisms underlying the activation of quiescent adult stem cells remain elusive. Using skeletal muscle stem cells, also called satellite cells (SCs), we demonstrate prevalent intron retention (IR) in the transcriptome of quiescent SCs (QSCs). Intron retained transcripts found in QSCs are essential for fundamental functions including RNA splicing, protein translation, cell cycle entry, and lineage specification. Further analysis reveals that phosphorylated-Dek protein modulates IR during SC quiescence exit. While Dek protein is absent in QSCs, Dek overexpression in vivo results in a global decrease of IR, quiescence dysregulation, pre-mature differentiation of QSCs, and undermined muscle regeneration. Moreover, IR analysis on hundreds of public RNA-seq data shows that IR is conserved among quiescent adult stem cells. Altogether, we illustrate intron retention as a conserved post-transcriptional regulation mechanism that plays an important role during stem cell quiescence exit.

Date of Award	2020
Original language	English
Awarding Institution	The Hong Kong University of Science and Technology